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Title Karakterisasi Antosianin Buah Murbei Spesies Morus alba dan Morus cathayana di Indonesia.
Call Number
ISBN/ISSN 2338-0950
Author(s) Tatas H.P. Brotosudarmo
Leenawaty Limantara
Rehmadanta Sitepu
Subject(s) Anthocyanin
Morus alba
Morus cathayana
Series Title
GMD Jurnal Ilmiah Nasional
Language Indonesia
Publisher Department of Mathematic and Natural Science Tadulako University
Publishing Year 2016
Publishing Place Palu
Abstract/Notes Mulberry fruit has been known to be rich in antioxidants due to its anthocyanin contents. Unfortunately, the utilization of mulberry fruits as a source of antioxidants and therapy is rare in Indonesia. Mulberry plants are only used for feeding domesticated animal or making tea from its leafs. This study aims to characterize anthocyanin of Morus alba and Morus cathayana. Characterization of anthocyanin exctracts from Morus alba and Morus cathayana were performed using solvent 0.1% hydrochloric acid (HCl) in methanol, and then analized by UV-Vis spectrophotometer and High Performance Liquid Chromatography (HPLC). The results of UV-visible absorption spectra obtained from the crude extract shows that absorption spectra of mulberry extract M. cathayana provide higher absorbance ± 1.3 a.u. compared to absorbance which was obtained from the crude extract of mulberry M. alba
are on ± 0.4 a.u. The total anthocyanin obtained from extract of M. cathayana, is 40,39 ± 7,64 mg/g dry weight compared with M. alba which has a value 11,57 ± 3,02 mg/g dry weight. Results of chromatogram of HPLC using XR-ODS column with elution A was 0.1% formic acid in acetonitrile and elution B was 0.1% formic acid in water shows both M.cathayana and M. alba have two dominant pigments of anthocyanin. However, the intensity of chromatograms obtained from M. cathayana is higher than the intensity of chromatograms of M. alba. The intensity of chromatogram of M. cathayana is 50 mAU on retention time 7.86 minutes and 15 mAU on retention time 8.38 minutes. The intensity of chromatogram of M. alba is 10 mAU on retention time 7.35 minutes and 3 mAU on retention time 7.76 minutes. The two dominant anthocyanins are predicted as cyanidin-3-O-glucoside and cyanidin-3-rutinoside.
Specific Detail Info Natural Sciences: Journal of Science and Technology. Vol. 5, No. 2, Agustus 2016.
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