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Title Molecular Analysis of Gene Expression Related to The Effects of DLBS3233 Treatment in Differentiation of 3T3-L1 Pre-Adipocyte
Edition
Call Number
ISBN/ISSN 0975-1491
Author(s) Rehmadanta Sitepu
Debbie Sofie Retnoningrum
Raymond R. Tjandrawinata
Subject(s) DLBS3233
Adipogenesis
Gene expression analysis
Real-time RT-PCR
Classification
Series Title
GMD Jurnal Ilmiah Internasional
Language English
Publisher Innovare Academic Sciences Pvt Ltd
Publishing Year 2016
Publishing Place Indore, India
Collation
Abstract/Notes Objective: DLBS3233 is a standardized extract combination containing Lagerstroemia speciosa and Cinnamomum burmannii. The effect of DLBS3233 on adipocyte differentiation was examined in this study.

Methods: 3T3-L1 pre-adipocyte was used to investigate gene expression using the real-time reverse transcription polymerase chain reaction (RTPCR)method. Oil red-O staining for detecting lipid formation was also carried out in this experiment.

Results: DLBS3233 caused cell differentiation of 3T3-L1 pre-adipocytes into adipocytes which were indicated by positive results on staining cells with oil red-O on day 6 of the differentiation process. Analysis of gene expressions associated with adipogenesis (C/EBP-α, PPAR-γ, C/EBP-δ, FASn and adiponectin) showed an increase compared to control. In this study, DLBS3233 at a concentration of 5 μg/ml exhibited better differentiation
effect than DLBS3233 at a concentration of 10 μg/ml.

Conclusion: DLBS3233 can stimulate differentiation of 3T3-L1 pre-adipocytes into adipocytes
Specific Detail Info International Journal of Pharmacy and Pharmaceutical Sciences Vol. 8, Issue 5, 2016
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