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Title Analysis on the Chlorophyll Content of Commercial Green Leafy Vegetables
Edition
Call Number
ISBN/ISSN
Author(s) Tatas H.P. Brotosudarmo
Leenawaty Limantara
Indriatmoko
Renny Indrawati
Martin Dettling
Subject(s) Chlorophyll
HPLC
leafy vegetable
nitrogen meter
SPAD
Classification
Series Title
GMD Prosiding Ilmiah Internasional
Language English
Publisher Sciencedirect
Publishing Year 2015
Publishing Place Singapore
Collation
Abstract/Notes The objectives of the present study were to evaluate the chlorophyll content of green leafy vegetables found commercially and carry out a comparative investigation between in vivo and in vitro data. The chlorophyll of green leafy vegetable can be used as visible parameters of the quality of vegetables during storage, since it will be degraded gradually along with post-harvest senescence. Therefore, the development of reliable in vivo chlorophyll measurement should be advantageous rather than visual observation for the purpose of quality control and product sortation. Here, the existence of chlorophylls in ten green leafy vegetables were reported as SPAD values of a handheld SPAD-502 chlorophyll meter and % N of an Agriexpert CCN-6000 nitrogen meter (in vivo data), as well as total peak area data of HPLC measurement for chlorophyll a and b after exhaustive extraction using methanol (in vitro data). Both in vivo and in vitro measurement gave comparable grouping of vegetables with high and low content of chlorophyll. Moreover, correlation plots between SPAD values and total peak area of HPLC showed adequate linear correlation (R2 > 0.7), revealing the potency of in vivo observation for the prediction of actual chlorophyll content in commercial leafy vegetables. SPAD values and % N presented strong linear relationship (R2 > 0.9), in which SPAD-meter performed better detection at very low values. The calibration curve for each species of vegetable should be substantial to overcome the limiting factors of in vivo observation, such as leaf size, tissue thickness, and variation of chloroplast distribution
Specific Detail Info Procedia Chemistry Vol. 14 (2015) Hal. 225-231
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