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Title Separation of Photosynthetic Pigments by High-Performance Chromatography: Comparison of Column Performance, Mobile Phase, and Temperature
Edition
Call Number
ISBN/ISSN
Author(s) Tatas H.P. Brotosudarmo
Leenawaty Limantara
Indriatmoko
Yuzo Shioi
Subject(s) Cholesteryl bonded
HPLC column
monolithic packing
particulate packing
photosynthetic pigments
reverse phase
Classification
Series Title
GMD Jurnal Ilmiah Internasional
Language English
Publisher Springer India, in co-publication with Indian Academy of Sciences
Publishing Year 2015
Publishing Place Singapore
Collation
Abstract/Notes High-performance liquid chromatography (HPLC) has been commonly used as method of separating and identifying photosynthetic pigments such as chlorophylls and carotenoids because of such advantages as speed, high resolution and sensitivity. In this technique, high separation relies largely on the type of column material. This study compared the efficiency of five reverse-phase columns, C8, C18, C18 monolithic, π-NAP, and cholester, for separation of photosynthetic pigments at several fixed conditions of mobile phase and temperature. This investigation also analysed the parameters of ΔtR and tR ratio for selected pigments and resolution for structural isomers, such as α- and β-carotene. Among above columns tested, cholester column is suitable for separation of pigments not only for a broad range of polarity, but also for hydrophobic pigments in a simple mobile phase. This finding can help in the selection of column and HPLC parameters in separating photosynthetic pigments
Specific Detail Info Procedia Chemistry Vol. 14 (2015) Hal. 202-201
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