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Title Composition of Photosynthetic Pigments in Red Alga Kappaphycus Alvarezi Cultivated at Different Depths
Edition
Call Number
ISBN/ISSN
Author(s) Tatas H.P. Brotosudarmo
Leenawaty Limantara
Heriyanto
Indriatmoko
Subject(s) Seaweeds
HPLC
Pigments
Red Algae
chromatography analysis
Classification
Series Title
GMD Prosiding Ilmiah Internasional
Language English
Publisher MRCPP
Publishing Year 2014
Publishing Place Malang
Collation
Abstract/Notes The red alga Kappaphycus alvarezii has been introduced and mono cultivated in Indonesia as a seaweed commodity. This species is specifically grown in shallow and clear seawater, although there are several reports concerning the cultivation in deep seawater. It is interesting to know compositional changes of chlorophylls and carotenoids when K. alvarezii is grown at different depths. We, therefore, cultivated K. alvarezii green and brown variants at about 0.2-m (normal grown condition), 1-m, and 2-m depths and successfully obtained different ratios of chlorophyll and carotenoid composition at different depths. Quantitative analyses of chlorophylls to carotenoids ratio were carried out using data of chromatogram peak area. We subsequently evaluated the photo- and thermo-stability of the pigment extracts to examine the effects of pigment composition on the degradation rate of the pigments. The photo-stability assay was conducted under 1417 µmol photons m-2•s-1 approximately for 45 min and recorded using a Spectrophotometer MultiSpec-1501 (Shimadzu, Kyoto, Japan). Thermo-stability assay was performed using a water bath at 90°C for 10, 20, 30, 60, 90, and 120 min and measured by Spectrophotometer UV-1700 (Shimadzu). This investigation is aimed to provide information regarding compositional change of the pigments by acclimation in terms of cultivation depths and pigment stability at condition of natural pigment composition in this alga
Specific Detail Info Humbolt Kolleg II in Conjuction with The 2nd International Conference on Natural Sciences (ICONS) 2014, Malang, 25-28, September 2014
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